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SCIENCE CHINA Life Sciences, Volume 61, Issue 9: 1115-1117(2018) https://doi.org/10.1007/s11427-018-9291-2

Intersectional gene inactivation: there is more to conditional mutagenesis than Cre

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  • ReceivedFeb 28, 2018
  • AcceptedMar 15, 2018
  • PublishedMay 18, 2018

Abstract

There is no abstract available for this article.


Funded by

grants RO1 DE022363 and RO1 DE022378 from the National Institute of Dental and Craniofacial Research.


Acknowledgment

I thank members of my laboratory for helpful discussions and critical reading of the manuscript. I apologize to colleagues whose work I have been unable to cite due to space limitations. Work in my laboratory is supported by grants RO1 DE022363 and RO1 DE022778 from the National Institute of Dental and Craniofacial Research.


Interest statement

The author declares that he has no conflict of interest.


References

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  • Figure 1

    Intersectional gene inactivation. A, Using the example of craniofacial development, an E10.5 embryo includes multiple neural crest cell-derived structures (first panel), including the Lateral Nasal Process (purple), Medial Nasal Process (blue), Maxillary Process (gold) and Mandibular Process (Orange). B, In a hypothetical example, Flpo driven by promoter 1 (top) may be expressed in the Medial- and Lateral Nasal Processes (A, second panel), and Cre driven by promoter 2 (middle) can be expressed in the Medial Nasal and Mandibular Processes (A, third panel); expression of Cre is prevented by a stop sequence flanked by Frt sites. Cre is therefore only expressed in domains where both promoter 1 and promoter 2 are active, resulting in conditional mutagenesis of a conventional floxed allele (bottom) in the Medial Nasal Process alone (A, fourth panel, yellow). Dre or ΦC31o could equally be used instead of Flpo for conditional activation of an appropriately designed Cre allele (containing Rox or AttP/B sites, respectively). Scanning electron micrographs taken from Facebase (https://www.facebase.org/).

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