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SCIENCE CHINA Materials, Volume 58, Issue 4: 294-301(2015) https://doi.org/10.1007/s40843-015-0044-3

Facile synthesis of liposome/Cu2xS-based nanocomposite for multimodal imaging and photothermal therapy

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  • ReceivedMar 5, 2015
  • AcceptedApr 10, 2015
  • PublishedApr 24, 2015

Abstract

A kind of multifunctional perfluoropentane (PFP) and ultrasmall Cu2−xS nanodots (u-Cu2−xS NDs) co-incorporated liposome (PFP@u-Cu2−xS NDs@liposome) nanocomposite has been facilely and successfully synthesized for enhanced ultrasound/infrared thermal/photoacoustic multimodal imaging and photothermal therapy upon near infrared (NIR) laser irradiation. Such a liposome-based nanocomposite possesses a number of advantages, such as high dispersity and stability, excellent biocompatibility, small particle size (<100 nm), well-defined core/shell structure, strong NIR absorption and photo-triggered vaporization of PFP, etc. The detailed in vitro investigations demonstrate that the as-synthesized PFP@ u-Cu2−xS NDs@liposome nanocomposite is capable of enhancing the contrasts of ultrasound/infrared thermal/photoacoustic multimodal imaging, and substantially improving the photothermal therapeutic efficacy. This novel liposome-based theranostic nanoplatform shows great potentials in the future cancer diagnosis and therapy.


Acknowledgment

This work was supported by the National Basic Research Program of China (2011CB707905), China National Funds for Distinguished Young Scientists (51225202), and


Interest statement

The authors declare that they have no conflict of interest.


Contributions statement

Shi J and Chen H initiated and guided the work. Mou J designed and conducted the experiments. Chen Y, Ma M, Zhang K gave useful suggestions. Wei C set up the experimental devices.


Author information

Juan Mou received her BSc degree at China University of Geosciences (Wuhan) (2007). She is now a PhD candidate at Shanghai Institute of Ceramics, Chinese Academy of Sciences (SICCAS). Her research interest includes the design, synthesis and biomedical applications of novel photothermal and photodynamic therapy materials.


Hangrong Chen received her PhD degree in SICCAS (2001). She is now a professor of SICCAS. Her research areas include the synthesis of mesoporous materials, multifunctional inorganic biomedical nanomaterials, and novel environmental catalytic materials. She has published more than 150 scientific papers which have been cited more than 4600 times by other scientists with an h-index of 38 (2014).


Jianlin Shi received his PhD degree in SICCAS (1989). He is now a professor of SICCAS. His research areas include the synthesis of mesoporous materials, mesoporous-based nano-composites, and their catalytic, biomedical and optical applications. He has published more than 300 scientific papers which have been cited more than 12,000 times by other scientists with an h-index of 59 (2014).


Supplement

Supplementary information

Experimental details are available in the online version of the paper.


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  • Scheme 1

    Schematic illustration of the as-synthesized PFP@u-Cu2−xS NDs@liposome for enhanced ultrasound/infrared thermal/photoacoustic multimodal imaging and photothermal therapy under NIR laser irradiation.

  • Figure 1

    TEM images of blank liposome (a) and oleylamine-capped u-Cu2−xS NDs (b). STEM (c) and HAADF-STEM image (d) of the PFP@u-Cu2−xS NDs@liposome. (e) UV-Vis absorption spectra of PFP@u-Cu2−xS NDs@liposome dispersions at different Cu2+ concentrations and (f) the corresponding linear fitting plots of absorbance vs. Cu2+ concentration at 1064 nm.

  • Figure 2

    Optical microscopic images of u-Cu2−xS NDs@liposome (a, b) and PFP@u-Cu2−xS NDs@liposome dispersion immersed in hot water (60°C) (c, d), and exposed to NIR laser irradiation (980 nm, 1.41 W cm−2, 5 min) (e, f).

  • Figure 3

    Ultrasound images of PFP@liposome (a, b) and PFP@u-Cu2−xS NDs@liposome dispersion (c, d) before and after NIR laser irradiation for 5 min. Infrared thermal images (e–h) of water droplet and aqueous droplets containing PFP@u-Cu2−xS NDs@liposome (Cu2+ concentration, C1: 12.5 ppm, C2: 50 ppm) under NIR laser irradiation at different time intervals. (i) Linear plot of photoacoustic signal intensity vs. Cu2+ concentration, the inset shows the corresponding photoacoustic images of agar gel cylinders containing PFP@u-Cu2−xS NDs@liposome at varied Cu2+ concentrations. (j) Temperature elevations of the PFP@u-Cu2−xS NDs@liposome dispersion exposed to NIR laser irradiation (980 nm, 1.41 W cm−2) vs. Cu2+ concentration.

  • Figure 4

    Optical microscopic images (a–d) and CLSM images (e–h) of HeLa cells stained with trypan blue, calcein-AM and prodium iodide (PI) after different treatments: (a, e) without treatment as a control; (b, f) treated with PFP@u-Cu2−xS NDs@liposome alone; (c, g) treated with NIR laser irradiation alone; (d, h) treated with the PFP@u-Cu2−xS NDs@liposome and NIR laser irradiation (980 nm, 1.41 W cm−2) together. All the scale bars in (a–h) are 50 μm.

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