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SCIENCE CHINA Materials, Volume 59, Issue 8: 665-674(2016) https://doi.org/10.1007/s40843-016-5077-0

Gold nanoparticle-based strip sensor for multiple detection of twelve Salmonella strains with a genus-specific lipopolysaccharide antibody

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  • ReceivedJun 6, 2016
  • AcceptedJul 12, 2016
  • PublishedAug 18, 2016

Abstract

In this study, an innovative competitive immunochromatographic strip sensor was developed for rapid detection of Salmonella based on a genus-specific anti- lipopolysaccharide (LPS) monoclonal antibody (mAb) and the heterogeneous coating antigen of a LPS-bovine serum albumin conjugate. Gold nanoparticles labeled anti-LPS mAb specifically reacted with the conserved outer core of the Salmonella LPS in the sample and the color formed on the T line was negatively correlated with the number of Salmonella cells. The sensitivity of Ra mutant LPS (without O-specific chains but has the conserved outer core) was 25 ng mL–1, which explained the detection of Salmonella at the genus level. Based on the gray values on the test line, the limit of detection of Salmonella was 103 colony-forming unit (CFU) for all twelve typical strains of Salmonella. The analysis of common Gram-negative and Gram-positive bacteria demonstrated that the strip assay was specific to Salmonella. A milk sample test showed that Salmonella at a low level (1–5 CFU mL–1) was detected without complex biochemical confirmation steps, sophisticated instruments and professional training.


Funded by

The National Natural Science Foundation of China(21471068)

National Key Technologies R&D Program from Ministry of Science and Technology of China(2012BAK08B01)

Special Fund for Argo-scientific Research in the Public Interest(201513006)

Natural Science Foundation of Jiangsu Province(BK201501,BK20140003,BE2013613,BE2013611,CSE11N1310)

Graduate Innovation Project in Jiangsu Province of China(KYLX15_1137)


Acknowledgment

This work was supported by the National Natural Science Foundation of China (21471068), the National Key Technologies R&D Program from Ministry of Science and Technology of China (2012BAK08B01), Special Fund for Argo-scientific Research in the Public Interest (201513006), the Natural Science Foundation of Jiangsu Province (BK201501, BK20140003, BE2013613, BE2013611 and CSE11N1310), and the Graduate Innovation Project in Jiangsu Province of China (KYLX15_1137).


Interest statement

The authors declare that they have no conflict of interest.


Contributions statement

Kuang H and Xu C conceived and designed the experiments. Wang W, Liu L, and Song S performed the experiments. Xu L, Kuang H and Zhu J analyzed the data. Wang W wrote the paper. Kuang H, Xu L, and Xu C revised and approved the final version of the paper. All authors reviewed the manuscript.


Author information

Wenbin Wang is now a PhD candidate at Jiangnan University. His research is focused on the development of monoclonal antibodies and biosensors of foodborne pathogens.


Hua Kuang is a professor of Jiangnan University. She received her PhD degree from China Agricultural University in 2009, and then joined Jiangnan University. Her research areas include nanoparticle assemblies for biosensors.


Supplement

Appendix

Supplementary information is available in the online version of this article.


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  • Figure 1

    Scheme of the immunochromatographic strip sensor for Salmonella spp. Weakened red color on the T line compared with that of the negative control and a red band on the C line indicated that the sample was positive for Salmonella.

  • Figure 2

    Detection of Ra LPS with the mAb 5H12 based competitive Immunochromatographic strip sensor (a). Standard curve of the competitive ELISA with the mAb 5H12 (b).

  • Figure 3

    (a) Images of the Immunochromatographic strip sensor for detection of twelve typical strains of Salmonella; (b) gray values of the T lines for detection of the twelve typical strains.

  • Figure 4

    Specificity of the immunochromatographic strip sensor with the Gram-negative and Gram-positive bacteria.

  • Figure 5

    Analysis of Salmonella at low level in pure milk sample after 12 h enrichment: 0, Negative control; 1, S. paratyphiA; 2, S. typhimurium; 3, S. enteritidis; 4, S. arizona.

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