SCIENCE CHINA Materials, Volume 60, Issue 4: 361-367(2017) https://doi.org/10.1007/s40843-016-9015-9

Unique cytological behavior of MC3T3-E1 osteoblasts on H2O2-modified C/C composites in vitro

More info
  • ReceivedDec 20, 2016
  • AcceptedFeb 23, 2017
  • PublishedMar 14, 2017


MC3T3-E1 osteoblasts were cultured on H2O2-modified and unmodified carbon/carbon (H-C/C and C/C) composites for one week in order to evaluate differences in cell adhesion, spreading, and proliferation. The results indicated a certain degree of enhancement in the cell adhesion capability of osteoblasts cultured on H-C/C samples. Cellular morphologies after cell adhesion were observed via scanning electron microscopy (SEM), which showed that the cells adhered more closely and spread more widely on the H-C/C sample surface. However, no cell appeared in several multiple and continuous types of minor pores on both the C/C and H-C/C surfaces. In addition, two unique situations were observed on the H-C/C samples: an outline change of the osteoblasts was observed when the cells spread across some minor pores, and the cells entered and adhered well in some larger pores.

Funded by

National Natural Science Foundation of China(51472203,51521061)

Natural Science Basic Research Plan in Shaanxi Province of China(2014JM6233)

“111” project of china(B08040)


This work was supported by the National Natural Science Foundation of China (51472203 and 51521061), the Natural Science Basic Research Plan in Shaanxi Province of China (2014JM6233) and “111” project of china (B08040).

Interest statement

The authors declare that they have no conflict of interest.

Contributions statement

Cao S and Li H designed the experiment; Cao S and Zhang L engineered the samples; Cao S cultured the cells and carried out the tests; Cao S wrote the paper with support from Lu J. All authors contributed to the general discussion.

Author information

Sheng Cao is a graduate student pursuing his PhD degree under the supervision of Prof. Hejun Li at Northwestern Polytechnical University, Xi’an, China. His PhD research focus is on the biomedical application of carbon/carbon composites.

Hejun Li is a professor supported by the National Science Fund for Distinguished Young Scholars. He is now the Dean of the School of Materials Science and Engineering in Northwestern Polytechnical University. His current research interests include advanced carbon/carbon composites, paper based friction materials, nanomaterials and liquid solid extrusion and other aspects of the research.


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