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SCIENTIA SINICA Chimica, Volume 48 , Issue 3 : 277-281(2018) https://doi.org/10.1360/N032017-00195

Progress in biomolecular cryo-electron microscopy

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  • ReceivedNov 24, 2017
  • AcceptedDec 27, 2017
  • PublishedJan 26, 2018

Abstract

Biomolecular structure is the basis of discovering life procedure on atom level, exploring disease mechanism, and developing targeting drugs. The main techniques to resolve biomolecular structure are X-ray crystallography, NMR spectroscopy, and cryo-electron microscopy (Cryo-EM). Recently, there was dramatical progress in Cryo-EM, the resolution of which reached atom level (ca. 3 Å). The Nobel Prize in Chemistry 2017 was awarded to three scientists for developing cryo-electron microscopy for the high-resolution structure determination of biomolecules in solution. This paper introduced the scientific background and application perspective of cryo-electron microscopy.


Funded by

国家自然科学基金(编号:,21735007,21475146)

国家重点基础研究发展计划(编号:,2017YFA0505400)


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  • 表 1   X-ray、NMR和EM技术发展相关的诺贝尔奖

    获奖年度

    获奖者

    奖项

    技术

    1912

    M. Laue

    物理学奖

    X-ray

    1915

    W.H. Bragg,

    W.L. Bragg

    物理学奖

    X-ray

    1962

    M. Perutz,

    J. Kendrew

    化学奖

    X-ray

    1964

    D. Hodgkin

    化学奖

    X-ray

    1985

    J. Karle,

    H. Hauptman

    化学奖

    X-ray

    1988

    J. Deisenhofer,

    R. Huber,

    H. Michel

    化学奖

    X-ray

    1946

    Rabi

    物理学奖

    NMR

    1952

    F. Bloch,

    E. Purcell

    物理学奖

    NMR

    1991

    R. Ernst

    化学奖

    NMR

    2002

    K. Wuthrich

    化学奖

    NMR

    2003

    P. Mansfield,

    P. Lauterbur

    生理与医学奖

    NMR/MRI

    1982

    A. Klug

    化学奖

    EM

    1986

    E. Ruska

    物理学奖

    EM

    2017

    J. Dubochet,

    J. Frank,

    R. Henderson

    化学奖

    Cryo-EM

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