SCIENTIA SINICA Vitae, Volume 49, Issue 9: 1076-1085(2019) https://doi.org/10.1360/SSV-2019-0161

Self-assembly molecular mechanism and modification function on material surface of hydrophobin HGFI

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  • ReceivedJul 29, 2019
  • AcceptedAug 12, 2019
  • PublishedSep 9, 2019


Hydrophobins are typically small and amphiphilic protein molecules produced by filamentous fungi in specific physiology conditions. These proteins can be self-assembled at the nanoscale forming hydrophobic and hydrophilic layer interfaces, which changes the properties of the material surface. This special ability of hydrophobins makes them widely applicable in various areas, such as surface modification, formation emulsions, protein purification, drug delivery and usage in biosensors. Herein, an overview of the research progress on hydrophobin is discussed. In particular, insights into the self-assembly molecular mechanism of hydrophobin HGFI, as specifically isolated from Grifola frondosa, and its applications in surface modification and drug sustain control release are elucidated.

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感谢中国农业农村部畜禽产品质量监督检验测试中心于雷、天津大学王泽方、山西太原理工大学牛宝龙、河北师范大学赵立强、诺维信(中国)生物技术有限公司宋东民、西北工业大学王翔翔和天津市第一中心医院杨玖霞等博士对本研究做出重要贡献. 感谢南开大学白艳玲教授、张秀明高工、孔德领教授、陈强教授、王恺副教授、天津医科大学龚珉教授、广东科伦药业有限公司丁靖志高工和天津科技大学梁俊教授等对本研究的贡献.


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  • Figure 1

    Hydrophobin layer structure showing class I (A) and class II (B) types of hydrophobins[4,43]

  • Figure 2

    General primary structure of a hydrophobin and the exact configuration of four disulfide bonds formed by eight cysteine residues

  • Figure 3

    Tertiary structure and self-assembly model of class II (A) and class I (B) hydrophobins[62,43]

  • Figure 4

    Application of hydrophobin HGFI dispersed multiwall carbon nanotubes (MWCNTs) on human IgG immobilization. A: Dispersed MWCNTs obtained using HGFI. B: immobilization of the complex of HGFI/MWCNTs and human IgG in Quartz Crystal Microbalance test. C: activity detection of human IgG adsorbed on HGFI/MWCNTs complex using gold nanoparticles labeled anti-human IgG antibody

  • Figure 5

    Application of hydrophobin HGFI modified polystyrene on carcinoembryonic antigen (CEA) immobilization. A: AFM image of uniform CEA antibody layer formed on coated HGFI. B: Standard curves of TR-IFMA assays using modified HGFI. (a) Modified, (b) unmodified, and (c) high-binding polystyrene microplates

  • Figure 6

    SEM images of the biofilm formed using Staphylococcus aureus ATCC 6538 on the surface of PCL films (small magnifications, A1–D1; large magnification, A2–D2); bare PCL film, (A1, A2); PCL modified by pH, (B1, B2); PCL modified by PA-1, (C1, C2); and PCL modified by HGFI, (D1, D2)[65]

  • Figure 7

    Scheme illustrating the transport of GLP-1 by hydrophobin and drug stability determination[19]

  • Figure 8

    Release curve of IND coated with hydrophobins in dynamic pH conditions[14]

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