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SCIENCE CHINA Life Sciences, Volume 60 , Issue 12 : 1396-1398(2017) https://doi.org/10.1007/s11427-017-9192-0

Both structure and function of human monoclonal antibodies contribute to enhancement of Zika virus infectivity in vitro

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  • ReceivedAug 2, 2017
  • AcceptedSep 11, 2017
  • PublishedNov 10, 2017

Abstract

There is no abstract available for this article.


Funded by

National Key Program Project Grant of Ministry of Science and Technology of China(2016YFC1201000)

Pioneer Program Project Grant of CAS(XDBP 030405)

grants from the Municipal Science and Technology Bureau Foundation of Guangzhou(2014Y2-00550,201508020263)


Acknowledgment

The work was supported by National Key Program Project Grant of Ministry of Science and Technology of China (2016YFC1201000), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDBP 030405) and grants from the Municipal Science and Technology Bureau Foundation of Guangzhou (2014Y2-00550, 201508020263).


Interest statement

The author(s) declare that they have no conflict of interest.


Supplement

SUPPORTING INFORMATION

Table S1 Characteristics of human monoclonal antibodies used in the study

The supporting information is available online at http://life.scichina.com and https://link.springer.com. The supporting materials are published as submitted, without typesetting or editing. The responsibility for scientific accuracy and content remains entirely with the authors.


References

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  • Figure 1

    The structural and functional basis of mAbs capable of enhancing ZIKV infection in vitro. A, K562 cells were infected with ZIKV in the presence or absence of serially diluted human monoclonal antibodies Mers-4 (anti-MERS-Co), ZK2B10 (anti-ZIKV-EDIII) and ZK8-4 (anti-ZIKV-DI/DII). Cells were harvested and stained intracellularlly with a pan-flavivirus antibody 4G2, and then analyzed by flow cytometry. B, Fold enhancement was analyzed by comparison to the percentage of infected cells in the presence or absence of antibodies. Antibodies were classified into four groups according to their binding sites on ZIKV soluble envelope protein. FL, fusion loop; EDI, domain I of envelope protein; EDII, domain II of envelope protein; EDIII, domain III of envelope protein. C, The Log10 IC50 value was plotted against the corresponding Log10 concentration of antibody at which the peak ADE was reached for ZIKV (n=14) infections. The R value and P value were calculated by linear regression analysis using GraphPad Prism 6.0 software.

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