SCIENCE CHINA Life Sciences, https://doi.org/10.1007/s11427-020-1783-9

Comparative study on virus shedding patterns in nasopharyngeal and fecal specimens of COVID-19 patients

More info
  • ReceivedMay 24, 2020
  • AcceptedJul 26, 2020
  • PublishedAug 5, 2020


There is no abstract available for this article.

Funded by

the Strategic Priority Research Program of the Chinese Academy of Sciences(CAS)

the National Natural Science Foundation of China(NSFC)

and Hebei Key Research and Development Program(20277704D)

Youth Innovation Promotion Association of CAS(2017122)


This work was supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (CAS) (XDB29010102 and XDA19090118), the National Natural Science Foundation of China (NSFC) (32041010), and Hebei Key Research and Development Program (20277704D). Y.B. is supported by the NSFC Outstanding Young Scholars (31822055) and Youth Innovation Promotion Association of CAS (2017122).

Interest statement

The author(s) declare that they have no conflict of interest.



The supporting information is available online at https://doi.org/10.1007/s11427-020-1783-9. The supporting materials are published as submitted, without typesetting or editing. The responsibility for scientific accuracy and content remains entirely with the authors.


[1] Jiang X., Luo M., Zou Z., Wang X., Chen C., Qiu J.. Asymptomatic SARS-CoV-2 infected case with viral detection positive in stool but negative in nasopharyngeal samples lasts for 42 days. J Med Virol, 2020, : jmv.25941 CrossRef PubMed Google Scholar

[2] Pan Y., Zhang D., Yang P., Poon L.L.M., Wang Q.. Viral load of SARS-CoV-2 in clinical samples. Lancet Infect Dis, 2020, 20: 411-412 CrossRef Google Scholar

[3] Wang C., Horby P.W., Hayden F.G., Gao G.F.. A novel coronavirus outbreak of global health concern. Lancet, 2020, 395: 470-473 CrossRef Google Scholar

[4] World Health Organization. (2020). Coronavirus disease (COVID-19) outbreak. https://www.who.int/emergencies/diseases/novel-coronavirus-2019. Google Scholar

[5] Xiao F., Sun J., Xu Y., Li F., Huang X., Li H., Zhao J., Huang J., Zhao J.. Infectious SARS-CoV-2 in feces of patient with severe COVID-19. Emerg Infect Dis, 2020, 26: 1920-1922 CrossRef PubMed Google Scholar

[6] Zhou F., Yu T., Du R., Fan G., Liu Y., Liu Z., Xiang J., Wang Y., Song B., Gu X., et al. Clinical course and risk factors for mortality of adult inpatients with COVID-19 in Wuhan, China: a retrospective cohort study. Lancet, 2020, 395: 1054-1062 CrossRef Google Scholar

[7] Zhu N., Zhang D., Wang W., Li X., Yang B., Song J., Zhao X., Huang B., Shi W., Lu R., et al. A novel coronavirus from patients with pneumonia in China, 2019. N Engl J Med, 2020, 382: 727-733 CrossRef PubMed Google Scholar

[8] Zou L., Ruan F., Huang M., Liang L., Huang H., Hong Z., Yu J., Kang M., Song Y., Xia J., et al. SARS-CoV-2 viral load in upper respiratory specimens of infected patients. N Engl J Med, 2020, 382: 1177-1179 CrossRef PubMed Google Scholar

  • Figure 1

    Virus dynamics in nasopharyngeal and fecal specimens of COVID-19 cases. A, The nasal-throat mixed swabs (n=66) and fecal samples (n=51) of all 23 cases were collected and detected by rRT-PCR. B, Sequential nasal-throat mixed swab (solid line) and fecal samples (dotted line) were collected in 11 cases and used for virus detection. Two critically ill cases were colored in red. The other nine cases with mild-to-moderate symptoms were colored in black. C, Virus detection of a family cluster includes the mother, father, and son. D, Comparative analysis on virus titers between the imported (red and blue) and secondary cases (green and cyan). E, The standard curve, produced by the pseudotyped virus with SARS-CoV-2 ORF1ab gene, for calculation of viral RNA copy numbers. The regression curve is y=–3.5691x+47.127 (R2=0.9993), where x represents the gradient dilution factor, y represents Ct values.

Copyright 2020  CHINA SCIENCE PUBLISHING & MEDIA LTD.  中国科技出版传媒股份有限公司  版权所有

京ICP备14028887号-23       京公网安备11010102003388号