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SCIENCE CHINA Life Sciences, https://doi.org/10.1007/s11427-020-1783-9

Comparative study on virus shedding patterns in nasopharyngeal and fecal specimens of COVID-19 patients

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  • ReceivedMay 24, 2020
  • AcceptedJul 26, 2020
  • PublishedAug 5, 2020

Abstract

There is no abstract available for this article.


Funded by

the Strategic Priority Research Program of the Chinese Academy of Sciences(CAS)

the National Natural Science Foundation of China(NSFC)

and Hebei Key Research and Development Program(20277704D)

Youth Innovation Promotion Association of CAS(2017122)


Acknowledgment

This work was supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (CAS) (XDB29010102 and XDA19090118), the National Natural Science Foundation of China (NSFC) (32041010), and Hebei Key Research and Development Program (20277704D). Y.B. is supported by the NSFC Outstanding Young Scholars (31822055) and Youth Innovation Promotion Association of CAS (2017122).


Interest statement

The author(s) declare that they have no conflict of interest.


Supplement

SUPPORTING INFORMATION

The supporting information is available online at https://doi.org/10.1007/s11427-020-1783-9. The supporting materials are published as submitted, without typesetting or editing. The responsibility for scientific accuracy and content remains entirely with the authors.


References

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  • Figure 1

    Virus dynamics in nasopharyngeal and fecal specimens of COVID-19 cases. A, The nasal-throat mixed swabs (n=66) and fecal samples (n=51) of all 23 cases were collected and detected by rRT-PCR. B, Sequential nasal-throat mixed swab (solid line) and fecal samples (dotted line) were collected in 11 cases and used for virus detection. Two critically ill cases were colored in red. The other nine cases with mild-to-moderate symptoms were colored in black. C, Virus detection of a family cluster includes the mother, father, and son. D, Comparative analysis on virus titers between the imported (red and blue) and secondary cases (green and cyan). E, The standard curve, produced by the pseudotyped virus with SARS-CoV-2 ORF1ab gene, for calculation of viral RNA copy numbers. The regression curve is y=–3.5691x+47.127 (R2=0.9993), where x represents the gradient dilution factor, y represents Ct values.

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